Fig. 5: Exploration of mouse ICC cell lines used to construct the footpad tumor LNM model.

A Schematic representation for the establishment of MuCCA1 footpad tumor model. B In vivo bioluminescence imaging was used to monitor primary footpad tumor and popliteal LN metastasis on Day 35 and Day 85. C Immunofluorescence staining of DAPI and β-catenin in MuCCA1 footpad tumor tissues. D Schematic representation for the establishment of mIC-23 footpad tumor model. E In vivo imaging used to monitor primary footpad tumor (black arrow) and popliteal LN metastasis (red arrow) on Day 21. F Immunofluorescence staining of DAPI, YAP, and β-catenin in AKT/YAP-induced iCCA sections and mIC-23 tumor tissues.