Fig. 2: Y RNA processing is independent of DROSHA, DICER, and RNase L.
From: PTBP1 protects Y RNA from cleavage leading to its apoptosis-specific degradation
A Expression of DROSHA and DICER in the Jurkat cells transfected with control siRNA (Ctrl.), siDROSHA, or siDICER by real-time PCR (n = 3) normalized by GAPDH (Left). Expression of Y RNA fragments in those cells, where apoptosis was induced by anti-Fas antibody (clone: CH-11), by northern blotting (Right). Upper panel, Y3 RNA and ASR; lower panel, Y5 RNA and ASR. B Dicer-knockout or wild-type (floxed Dicer) MEFs were cultivated with or without 10 µM staurosporine for 2 h, followed by the detection of Y3 RNA cleavage by northern blotting. C Expression of RNase L in the Jurkat cells transfected with control siRNA (Ctrl.) or siRNase L by real-time PCR (n = 3) normalized by GAPDH. Y3 RNA or Y5 RNA cleavage was also detected by northern blotting as in (A). D Rnasel-knockout or wild-type MEFs were stimulated with 10 μM staurosporine for 2 or 6 h, followed by the detection of Y3 RNA cleavage by northern blotting. Similar results were obtained in two independent experiments. Student’s t-test was used for the statistical analysis. *P < 0.05, error bars; mean ± s.d.
