Fig. 4: PTBP1 is responsible for the caspase 3-dependent Y RNA cleavage.
From: PTBP1 protects Y RNA from cleavage leading to its apoptosis-specific degradation
A Expression of PTBP1 in an equal volume of each IEC fraction analyzed by western blotting with anti-PTBP1 antibody. B Y3 RNA cleavage with or without recombinant PTBP1 (left). Y3 RNA/ASR ratios were calculated and normalized by the reaction without PTBP1 and plotted as a bar graph (right). C Cleavage of WT or caspase 3-resistant PTBP1 by caspase 3 by western blotting. D Y3 RNA cleavage detected by northern blotting under the condition of (C) (left). The cleavage activities were calculated as a ratio of Y3 RNA/cleaved products, then normalized by the reaction without caspase 3, respectively (right). E Schematic representation of the caspase 3-dependent Y RNA cleavage in apoptosis. Similar results were obtained in two independent experiments.
