Fig. 5: Schematic representation of the genome-edited sequences of Ptbp1mut/mut mice.
From: PTBP1 protects Y RNA from cleavage leading to its apoptosis-specific degradation
The point mutation at (i) D7A, (ii) D138A and D171A in Ptbp1 gene were introduced into oocyte of C57BL/6 mice by CRISPR/Cas9 system, respectively. Then these mice were inbred to generate (iii) D7A, D138A and D171A triple mutation knock-in mice (Caspase 3-resistant PTBP1 knock-in mice: Ptbp1mut/mut mice).
