Fig. 2: YTHDF3 silencing recovered CD8+ T antitumor activity to inhibit NSCLC immune evasion. | Cell Death Discovery

Fig. 2: YTHDF3 silencing recovered CD8+ T antitumor activity to inhibit NSCLC immune evasion.

From: YTH domain family protein 3 accelerates non-small cell lung cancer immune evasion through targeting CD8+ T lymphocytes

Fig. 2

A The YTHDF3 shRNAs were transfected into A549 cells and the silencing efficiency was detected using western blot. B The apoptosis of A549 cells were detected using flow cytometry. C The co-culture system was constructed using CD8+ T cells and NSCLC cells. D IFN-γ, E TNF-α, F Granzyme-B and G perforin secreted by CD8+ T cells were detected by ELISA kit upon co-cultured with YTHDF3 silencing transfected A549 cells. H The cytotoxicity was measured by LDH release assay after incubation with activated CD8+ T cells. I, J The cell-surface PD-L1 expression in NSCLC cells (A549) was detected by flow cytometry. *p < 0.05; **p < 0.01.

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