Fig. 3: YTHDF3 up-regulation impaired the CD8+ T antitumor activity to deteriorate immune evasion. | Cell Death Discovery

Fig. 3: YTHDF3 up-regulation impaired the CD8+ T antitumor activity to deteriorate immune evasion.

From: YTH domain family protein 3 accelerates non-small cell lung cancer immune evasion through targeting CD8+ T lymphocytes

Fig. 3

A The YTHDF3 overexpression plasmids were transfected into H1299 cells and the overexpression efficiency was detected using western blot. B The apoptosis of H1299 cells were detected using flow cytometry. C IFN-γ, D TNF-α, E Granzyme-B and F perforin secreted by CD8+ T cells were detected by ELISA kit upon co-cultured with YTHDF3 overexpression transfected H1299 cells. G The cytotoxicity was measured by LDH release assay after incubation with activated CD8+ T cells. H, I The cell-surface PD-L1 expression in NSCLC cells (H1299) was detected by flow cytometry. *p < 0.05; **p < 0.01.

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