Fig. 5: YTHDF3 enhanced the PD-L1 mRNA stability. | Cell Death Discovery

Fig. 5: YTHDF3 enhanced the PD-L1 mRNA stability.

From: YTH domain family protein 3 accelerates non-small cell lung cancer immune evasion through targeting CD8+ T lymphocytes

Fig. 5

A, B RIP assay using anti-m6A antibody was performed in NSCLC cells with YTHDF3 silencing (A) or YTHDF3 overexpression (B) to reflect the molecular interaction within YTHDF3 and PD-L1. C, D RNA stability analysis using RNA decay assay was performed to determine the half life time (t1/2) for PD-L1 mRNA with YTHDF3 silencing (A) or YTHDF3 overexpression (D). E The luciferase reporters with PD-L1 3′UTR wild-type (WT) sequences and corresponding mutant (Mut) with putative mutated m6A sites were constructed. F Luciferase activity assay was performed to detect the luciferase activity in A549 or H1299 cells with co-transfected with PD-L1-WT/Mutant plasmid. *p < 0.05; **p < 0.01.

Back to article page