Fig. 6: YTHDF3 targeted PD-L1 to repress CD8+ T antitumor activity to induce NSCLC immune evasion. | Cell Death Discovery

Fig. 6: YTHDF3 targeted PD-L1 to repress CD8+ T antitumor activity to induce NSCLC immune evasion.

From: YTH domain family protein 3 accelerates non-small cell lung cancer immune evasion through targeting CD8+ T lymphocytes

Fig. 6

A The YTHDF3 overexpression plasmids were transfected into H1299 cells and the overexpression efficiency was detected using western blot. (B-E) ELISA assays were performed to detect the (B) IFN-γ, (C) TNF-α, (D) Granzyme-B and (E) perforin secreted by CD8+ T cells co-cultured with H1299 cells transfected with YTHDF3 overexpression/si-PD-L1. F The cytotoxicity was measured by LDH release assay after incubation with activated CD8+ T cells. G, H The cell-surface PD-L1 expression in NSCLC cells (H1299) was detected by flow cytometry. *p < 0.05; **p < 0.01.

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