Fig. 4: FAM83A promoted LUSC cell proliferation via inhibiting its ferroptosis level. | Cell Death Discovery

Fig. 4: FAM83A promoted LUSC cell proliferation via inhibiting its ferroptosis level.

From: Family with sequence similarity 83, member A (FAM83A) inhibits ferroptosis via the Wnt/β-catenin pathway in lung squamous cell cancer

Fig. 4

A–C Colony formation and CCK-8 experiments were conducted in FAM83A-overexpression and -knockout groups. FAM83A overexpression increased the colony formation and cell proliferation capacity, while FAM83A knockdown inhibited its colony formation and cell proliferation capacity. D The ferroptosis features shown by the electron microscopy after FAM83A knockdown. The mitochondrial volume decreased, the double-membrane density increased, and the mitochondrial ridge decreased or disappeared in the siFAM83A group. E Western blot revealed that FAM83A downregulation inhibited glutathione peroxidase 4 (GPX4) expression, while GPX4 upregulated in FAM83A overexpression group. F FAM83A overexpression inhibited ferroptosis, including decreasing Fe2+, ROS, and MDA content and increasing GSH level, whereas the siFAM83A group induced the opposite results. G, H Colony formation and I CCK8 experiments revealed that FAM83A overexpression increased LUSC proliferation, while FIN56 counteracted its function.

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