Fig. 5: MIR155HG promotes CCL5 transcription through YBX1 in LUAD. | Cell Death Discovery

Fig. 5: MIR155HG promotes CCL5 transcription through YBX1 in LUAD.

From: The recruitment of CD8+ T cells through YBX1 stabilization abrogates tumor intrinsic oncogenic role of MIR155HG in lung adenocarcinoma

Fig. 5

A The human CCL5 proximal promoter (−1 to −2 kb) was divided into seven different segments. B ChIP analysis was used to study the potential binding of YBX1 in A549 cells. YBX1 bound to the designated regions (C1, containing the Y-box sequence). ChIP assays were performed using a YBX1-specific antibody, R-IgG was the ChIP control. C Three different luciferase vectors constructed according to CCL5 promoter C1 region were co-transfected with si-YBX1 into A549 cells. The relative firefly/Renilla luciferase activities were analyzed in the cells 24 h after transfection. D Increased CCL5 expression in MIR155HG-overexpressing A549 cells was abolished by YBX1 knockdown by qRT-PCR. E Increased secretion levels of CCL5 in MIR155HG-overexpressing A549 cells were abolished by YBX1 knockdown by ELISA assay. F Increased recruitment of CD3+CD8+ T cells in MIR155HG-overexpressing A549 cells was abolished by YBX1 knockdown by flow cytometry analysis. Results are presented as mean ± SEM, n = 3. *p < 0.05, **p < 0.01, ***p < 0.001.

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