Fig. 6: MIR155HG upregulates PD-L1 transcription to hamper the activity of recruited CD8+ T cells. | Cell Death Discovery

Fig. 6: MIR155HG upregulates PD-L1 transcription to hamper the activity of recruited CD8+ T cells.

From: The recruitment of CD8+ T cells through YBX1 stabilization abrogates tumor intrinsic oncogenic role of MIR155HG in lung adenocarcinoma

Fig. 6

A CCK8 assay detected the killing of tumor cells by indicated activated PBMC. A549 cells were co-cultured with/without PBMC for 24 h. B Activated PBMC were co-cultured with A549 cells with MIR155HG knockdown or overexpressed for 24 h. The PBMC were collected and stained with FITC-Annexin V, then subjected to flow cytometry analysis. C Quantification of apoptosis cells detected by flow cytometry. D qRT-PCR was performed to detect PRF1, GZMB, GNLY and IFNG in activated PBMC co-cultured with A549- sh-NC/sh-MIR155HG and A549- Vector /MIR155HG (E) for 48 h. F Flow cytometry analysis detected the intracellular IFNG level and IL-2 level (G) of CD3+CD8+ T cells in PBMC after co-culturing with A549-vector/MIR155HG cells for 3 days. H qRT-PCR analysis of PD-L1 mRNA in A549 cells and PC9 cells (I) with MIR155HG knockdown or overexpressed. J Western blot analysis of PD-L1 and GAPDH in A549 cells and PC9 cells (K) with MIR155HG knockdown or overexpressed. L Flow cytometry analysis detected PD-L1 in A549 cells with MIR155HG knockdown or overexpressed. Results are presented as mean ± SEM, n = 3. **p < 0.01, ***p < 0.001.

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