Fig. 1: MDH2 is downregulated in ccRCC and associated with its staging. | Cell Death Discovery

Fig. 1: MDH2 is downregulated in ccRCC and associated with its staging.

From: MDH2 regulates the sensitivity of clear cell renal cell carcinoma to ferroptosis through its interaction with FSP1

Fig. 1

A Expression of MDH2 in ccRCC and normal renal tissue according to TCGA database. B The mRNA level of MDH2 in ccRCC and adjacent normal tissues (n = 6). The expression level of β-Actin was used as normalized control. The statistical analysis comes from technical replicates and the experiment was repeated three times. Data was presented as mean ± SD. C The protein level of MDH2 and β-Actin in ccRCC and adjacent normal tissues (n = 6). The statistical analysis comes from technical replicates and the experiment was repeated three times. (T tumor tissue, N normal tissue). Data was presented as mean ± SD. D Representative images of MDH2 staining in ccRCC tumor tissues and normal tissues. E The IHC score of MDH2 in normal tissues and ccRCC tissues. F The IHC score of MDH2 in different histological grades of ccRCC tissues. G The MDH2 mRNA level in the normal kidney cell lines (HK2 and HKC8) and ccRCC cell lines (786-O and 769-P). The expression level of β-Actin was used as the normalized control. The statistical analysis comes from biological replicates and the experiment was repeated three times. Data was presented as mean ± SD. H The protein level of MDH2 in the normal kidney cell line (HK2 and HKC8) and ccRCC cell lines (786-O and 769-P), and β-Actin was used as control. The statistical analysis comes from biological replicates and the experiment was repeated three times. Data was presented as mean ± SD. (*P < 0.05, **P < 0.01, ***P < 0.001, and ns not significant.).

Back to article page