Fig. 4: MDH2 regulates the sensitivity of ferroptosis through the modulation of FSP1. | Cell Death Discovery

Fig. 4: MDH2 regulates the sensitivity of ferroptosis through the modulation of FSP1.

From: MDH2 regulates the sensitivity of clear cell renal cell carcinoma to ferroptosis through its interaction with FSP1

Fig. 4

A Protein expression levels of in ferroptosis key factor in MDH2 overexpressing and knockout ccRCC cells. The statistical analysis comes from biological replicates and the experiment was repeated three times. Data was presented as mean ± SD. B Response-dose curves of dual-knockout cells to RSL3 and erastin. The statistical analysis comes from biological replicates and the experiment was repeated three times. Data was presented as mean ± SD. C WB assay detected the knockout efficiency of FSP1. D Flow cytometry analysis of cell death in FSP1/MDH2 dual-knockout cells treated with RSL3 (0.5 μM, 6 h). The statistical analysis comes from technical replicates and the experiment was repeated three times. Data was presented as mean ± SD. E Flow cytometry analysis of lipid peroxidation in FSP1/MDH2 dual-knockout cells treated with RSL3 (0.1 μM, 6 h). The statistical analysis comes from technical replicates and the experiment was repeated three times. Data was presented as mean ± SD. (*P < 0.05, **P < 0.01, ***P < 0.001, and ns not significant).

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