Fig. 8: Bithionol causes ferroptosis in AML cells. | Cell Death Discovery

Fig. 8: Bithionol causes ferroptosis in AML cells.

From: Bithionol eliminates acute myeloid leukaemia stem-like cells by suppressing NF-κB signalling and inducing oxidative stress, leading to apoptosis and ferroptosis

Fig. 8

Mitochondrial ROS in KG-1a cells after 1 (A) and 24 (B) h of treatment with bithionol. C, D Effects of the selective ferroptosis inhibitor ferrostatin-1 on the death of KG-1a cells induced by bithionol. The cells were pretreated for 2 h with 1 μM ferrostatin-1 and then co-incubated with 28 μM bithionol for 72 h. Vehicle (0.2% DMSO) was used as a negative control (CTL), and hydrogen peroxide (H2O2, 100 µM) was used as a positive control. The data are shown as the mean ± SEM of three biological replicates carried out in duplicate. *p < 0.05 compared with CTL by one-way ANOVA followed by Dunnett’s multiple comparisons test. #p < 0.05 compared with the respective treatment without inhibitor by one-way ANOVA followed by Dunnett’s multiple comparisons test. MFI mean fluorescence intensity.

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