Fig. 3: HECTD3 facilitates IR-induced DNA damage repair.
From: Targeting the HECTD3-p62 axis increases the radiosensitivity of triple negative breast cancer cells
A HECTD3 KO reduced pCHK1 (S345) protenin levels. HECTD3 (Con), HECTD3 (KO) and p62 knockdown/HECTD3 KO HCC1806 and HCC1937 cells were irradiated with 10 Gy. CHK1 and pCHK1 were detected by WB. B HECTD3 KO HCC1806 and HCC1937 cells show a decrease of IR-induced γH2AX foci compared to control cells. At 0, 1, 6, 12 h after irradiation (10 Gy), the cells were immunostained with anti-γH2AX (red) and DAPI (blue) and analyzed using a confocal microscopy. The cells containing >10 γH2AX foci were recorded as γH2AX positive cells. Average percentage values from at least 100 cells were recorded. The data are displayed as the mean plus SD of three counts, and statistical significance was calculated and represented as the P-value. *P < 0.05; ns, not significant. Scale bars, 20 µm. C HECTD3 enhances DNA damage repair in HCC1806 and HCC1937 cells, as measured by the COMET assay. Cells were treated with IR (10 Gy) for 4 h or 12 h and COMET images were captured using a fluorescence microscopy. The tail moment was calculated as the tail length multiplied by the tail DNA percentage in at least 100 cells. The data are displayed as the mean plus SD of three counts, and statistical significance was calculated and represented as the P-value. *P < 0.05; ***P < 0.001 and ns, not significant. Scale bars, 100 µm.
