Fig. 6: The regulatory axis of NEAT1/miR-125b-5p/SLC1A5 and the effect of NEAT1 in HNSCC cells. | Cell Death Discovery

Fig. 6: The regulatory axis of NEAT1/miR-125b-5p/SLC1A5 and the effect of NEAT1 in HNSCC cells.

From: The disruption of NEAT1-miR-125b-5p-SLC1A5 cascade defines the oncogenicity and differential immune profile in head and neck squamous cell carcinoma

Fig. 6

A Illustration of NEAT1/miR-125b-5p/SLC1A5 regulatory axis. B Knockdown of NEAT1 expression by si-NEAT1. C The miR-125b-5p expression fold changes after 100 nM si-NEAT1 treatment. D The SLC1A5 protein expression following 100 nM si-NEAT1 or 100 nM si-SLC1A5 treatment. Lt, Western blot analysis. Rt, qPCR analysis to detect NEAT1 expression. E The SLC1A5 protein expression following 100 nM si-NEAT1 treatment with or without the treatment with 100 nM miR-125b-5p inhibitor. Numbers designate the normalized expression value. Prolonged image exposure is done in this diagram. F The GSH/GSSG ratio. After the treatment with 6.9 µM or 15.7 µM cisplatin in OECM1 or FaDu cells, respectively, for 24 hours, cells are treated with si-NEAT1, and the GSH/GSSG ratios are assayed. Mann-Whitney test. *, **, *** and ****, P < 0.05, P < 0.01, P < 0.001 and P < 0.0001, respectively.

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