Fig. 2: Apoptosis was activated by αCLDN18.2-MMAE in CLDN18.2-positive gastric cancer cells. | Cell Death Discovery

Fig. 2: Apoptosis was activated by αCLDN18.2-MMAE in CLDN18.2-positive gastric cancer cells.

From: Enhancing antitumor efficacy of CLDN18.2-directed antibody-drug conjugates through autophagy inhibition in gastric cancer

Fig. 2

A, B MKN45-CLDN18.2 and SNU-601 cells were incubated with indicated concentrations of αCLDN18.2-MMAE for 48 h and then stained with Annexin V-FITC/PI to detect apoptosis by flow cytometry. The proportions of Annexin V-positive cells from repeat experiments were statistically calculated. Data were shown as mean ± S.D. (n = 3) and analyzed by ordinary one-way ANOVA (**P < 0.01). C, D After 48 h of treatment with varying concentrations of αCLDN18.2-MMAE, the expression levels of cleaved PARP and cleaved caspase-9 were determined through Western Blot analysis and quantified by ImageJ. The results were presented as mean ± S.D. (n = 3) and analyzed by ordinary one-way ANOVA (**P < 0.01).

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