Fig. 1: S. aureus elicits an IFN-I response in murine BMDMs.
From: Type 1 interferons promote Staphylococcus aureus nasal colonization by inducing phagocyte apoptosis
BMDMs were infected with S. aureus strains LAC USA300 or Newman at a MOI of 100 for 1 h. BMDMs were then incubated with gentamicin media for 1 h which was then replaced with antibiotic free media. At 24 h post gentamicin treatment, RNA was extracted from S. aureus infected BMDMs and analyzed using the myeloid panel on the Nanostring technologies nCounter system. Fold change from uninfected BMDMs was determined for Newman (A) and LAC USA300 (B) infected BMDMs using the Nanostring advanced analysis software, nSolver and graphed as volcano plots using Prism, Graphpad. Pathway analysis was also conducted for both strains (C) using nSolver which uses each gene’s first principal component to create a pathway score. Statistics for heatmap analysis was performed using a student’s t test comparing the means of log2 transformed normalized data. Genes with an FDR adjusted P value < 0.05 are highlighted red above the dotted horizontal black line.
