Fig. 3: S. aureus strain Newman shows significantly less intracellular survival and drives the expression of the proapoptotic genes trail and daxx in BMDMs.
From: Type 1 interferons promote Staphylococcus aureus nasal colonization by inducing phagocyte apoptosis
BMDMs were infected with S. aureus strain LAC USA300 or Newman at a MOI of 100 for 1 h. BMDMs were then incubated with gentamicin media for 1 h which was then replaced with antibiotic free media. 24 h post gentamicin treatment, cells were then lysed for CFU enumeration and presented as either Log(CFU/ml) (A) or fold change of Log(CFU/ml) at T0h (B). Cells were lysed for RNA isolation and then assessed using the Nanostring nCounter platform with the myeloid panel. Heatmap of extrinsic apoptosis related genes contained within the Nanostring panel (C). Log2(Normalized counts for trail (D) and daxx (E). Results are expressed as mean ± SD with CFU data statistically analyzed using a two-way ANOVA with a Šidák multiple comparisons posttest or for the Nanostring data using a one-way ANOVA with a Tukey post-test (P value * <0.05, ** <0.01, *** <0.001 and **** <0.0001) for n = 3–6 independent experiments.
