Table 1 Various dysregulated miRNAs with respect to PD-1/PD-L1 in lung cancer.
From: The miRNA and PD-1/PD-L1 signaling axis: an arsenal of immunotherapeutic targets against lung cancer
Sr.No. | miRNA | Regulation | Property | In-vitro and in-vivo systems used | Target | Validation methods | References |
|---|---|---|---|---|---|---|---|
1. | miR-4458 | downregulated | Tumor-suppressor | A549, H1299, SW900, LCC (mouse lung cancer cell line), normal human lung fibroblast cell line 2BS; C57BL/6 mice model | STAT3 | ELISA, Western blot, CCK-8 assay, Luciferase assay, qRT-PCR | [113] |
2. | miR-526b-3p | downregulated | Tumor-suppressor | BEAS-2B, A549, H1975, PC-9; A549 metaststic xenograft models (BALB/c nude mice) | STAT3 | miRNA microarray analysis, MTT assay, Migration assay, Western blot, qRT-PCR, Luciferase assay, IHC | [118] |
3. | miR-34a | downregulated | Tumor-suppressor | A549, H460, H1299; 344SQ syngeneic mouse model, H1299 xenograft model | PD-L1 | IHC, Flow cytometry qRT-PCR, Western blotting, Luciferase assay | [119] |
4. | miR-200b | downregulated | Tumor-suppressor | 393P, 393LN, 344P, 344SQ, 531LN2, 531LN3, LLC-JSP Lewis lung cell lines; 129/Sv mice | ZEB1 | Immunohistochemistry, Flow-cytometry, qRT-PCR, Luciferase assay | [126] |
5. | miR-16-5p | downregulated | Tumor-suppressor | A549, PC9, HCC827; BALB/c nude male mice | PD-L1 | IHC, Western blot, Colony formation assay, Wound healing assay, Flow cytometry, qRT-PCR | [130] |
6. | miR-320a | downregulated | Tumor-suppressor | A/J mice model | PD-L1 | IHC, Western blot, In-situ hybridization | [135] |
7. | miR-4315 | upregulated | Oncogenic | A172, MCF7, OV9, A172, A549; MNRI Nude mice | Bim | ELISA, Luciferase assay | [136] |
8. | miR-181a | downregulated | Tumor-suppressor | A549, H69, LL/2 cells; C57BL6/J mice model | STAT3 | qRT-PCR, Western blot, Flow cytometry, MTT assay | [141] |
