Fig. 4: The activated E2F1 up-regulated the transcription of Notch1, resulted in the activation of Notch pathway, and inhibition of Wnt pathway in p21G2TKO MEFs.
From: p21 Regulates Wnt-Notch balance via DREAM/MMB/Rb-E2F1 and maintains intestinal stem cell homeostasis
A Co-IP with anti-E2F1 antibody indicate that E2F1 binding Rb reduced in p21G2TKO cells. B ChIP assay with anti-E2F1 antibody reveals that E2F1 binds Notch1 promoter with higher efficiency in p21G2TKO cells, suggesting the released E2F1 activity promoted Notch1 transcription. N = 3, **, p < 0.01; ***, p < 0.001. C Co-IP with anti-β-Catenin antibody indicated more Notch1 and Rbpj proteins are bound to β-Catenin in p21G2TKO cells, suggesting that the up-regulation of Notch1 results in its enhanced binding to β-Catenin. D ChIP assay with anti-β-Catenin antibody showed that more β-Catenin bound to Hes1 promoter in p21G2TKO cells. N = 3, *, p < 0.05; **, p < 0.01.
