Fig. 2: Knockdown TMEM209 inhibits the proliferation and metastasis of HCC in vitro. | Cell Death Discovery

Fig. 2: Knockdown TMEM209 inhibits the proliferation and metastasis of HCC in vitro.

From: TMEM209 promotes hepatocellular carcinoma progression by activating the Wnt/β-catenin signaling pathway through KPNB1 stabilization

Fig. 2

A Knockdown efficiency of TMEM209 in MHCC-97H and Huh7 cells was evaluated by western blotting and qPCR. B The CCK-8 assays were performed to detect the effects of TMEM209 knockdown on the proliferation efficiency of MHCC-97H and Huh7 cells. C Representative images (upper) and statistical analysis (lower) showed the effects of TMEM209 knockdown on colony formation in MHCC-97H and Huh7 cells. D Representative images of EdU experiments (left) and statistical analysis (right) showed the cell proliferation efficiency in the indicated groups. E The effects of TMEM209 knockdown on migration (upper) and invasion (lower) were evaluated by the Transwell assays. (Scale bar = 200 μm). F Wound healing assays were performed to detect the effects of TMEM209 knockdown on the motility of MHCC-97H and Huh7 cells. G Protein levels of PCNA, Cyclin D1, E-CAD, N-CAD, and vimentin and corresponding statistical results for the indicated groups. The results are presented as the mean ± SD of three independent experiments. n.s. indicates no significant difference. *P < 0.05, **P < 0.01, ***P < 0.001.

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