Fig. 2: Inhibiting Nr1d1 expression mitigates CIH-induced PH development in rodents. | Cell Death Discovery

Fig. 2: Inhibiting Nr1d1 expression mitigates CIH-induced PH development in rodents.

From: Nr1d1 inhibition mitigates intermittent hypoxia-induced pulmonary hypertension via Dusp1-mediated Erk1/2 deactivation and mitochondrial fission attenuation

Fig. 2

A Histological evaluation of rat lung tissues containing peripheral pulmonary vascular in control group, CIH + AAV1-Luc group, and CIH + AAV1-Nr1d1 group through H&E staining, α-SMA immunohistochemistry, and Masson staining, scale bar = 10 µm. B Waveforms of right ventricular systolic pressure (RVSP) in three groups of rats. C Relative quantitative analysis of RVSP in three groups of rats. D Fulton index (right ventricle/ (left ventricle + septum) weight ratio) in three groups of rats. E Quantification of vascular medial wall thickness in image A. F The ratio of non-muscularized, partially muscularized, and fully muscularized pulmonary arterioles in CIH-induced PH rats. G Wheat germ agglutinin (WGA) staining on right ventricular tissues from three rat groups, scale bar = 20 µm. H Histological evaluation of mouse lung tissues containing peripheral pulmonary vascular in three mouse groups through H&E staining, α-SMA immunohistochemistry, and Masson staining, scale bar = 10 µm. I RVSP in three groups of mice. J Relative quantitative analysis of RVSP in three groups of mice. K Fulton index in three groups of mice. L Quantification of vascular medial wall thickness in image H. M The ratio of non-muscularized, partially muscularized, and fully muscularized pulmonary arterioles in CIH-induced PH mice. N WGA staining on right ventricular tissues from three mouse groups, scale bar = 20 µm. Involved 6 biological replicates (N = 6). Data are shown as mean ± SD; *P < 0.05 verse Control group; #P < 0.05 verse CIH + AAV1-Luc group.

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