Fig. 4: Nr1d1 deficiency mitigates IH-induced mitochondrial dysfunction and fission in PASMCs. | Cell Death Discovery

Fig. 4: Nr1d1 deficiency mitigates IH-induced mitochondrial dysfunction and fission in PASMCs.

From: Nr1d1 inhibition mitigates intermittent hypoxia-induced pulmonary hypertension via Dusp1-mediated Erk1/2 deactivation and mitochondrial fission attenuation

Fig. 4

A Representative flow cytometry images of Mito-SOX staining in three PAMSCs groups. B Summarized OCR tracings in PASMCs in indicated groups (n = 4 independent experiments). C The Basal OCR, maximum respiration, and ATP production in three PASMCs groups. D Image and relative quantitative analysis of mitochondrial membrane potential in various groups of PASMCs using the JC-1 kit, scale bar = 50 µm. E Representative confocal images of the mitochondrial network of PASMCs examined by MitoTracker Deep Red, and the number of individual mitochondria as well as the mean mitochondrial branch lengths were calculated, scale bar = 5 µm. Involved 4 biological replicates (N = 4). Data are shown as mean ± SD; *P < 0.05 verse Control group; #P < 0.05 verse IH group.

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