Fig. 5: Dusp1 is the direct trans-repression target of Nr1d1 in regulating mitochondrial function. | Cell Death Discovery

Fig. 5: Dusp1 is the direct trans-repression target of Nr1d1 in regulating mitochondrial function.

From: Nr1d1 inhibition mitigates intermittent hypoxia-induced pulmonary hypertension via Dusp1-mediated Erk1/2 deactivation and mitochondrial fission attenuation

Fig. 5

A Map of Nr1d1 enrichments relative to the body of RefSeq genes and to 1.5 kbp upstream of the TSS and 1.5 kbp downstream of the TES. X-axis in base-pairs. TSS = Transcription start site, TES = transcription end site. Y-axis average enrichment of CHIP-seq reads over genes and upstream and downstream regions. B Nr1d1 profile across the promoter region of Dusp1 on chromosome 5 in human PASMCs of the Nr1d1-Input or Nr1d1-Ip group. C, D ChIP-qPCR and quantification were conducted using Nr1d1 antibodies to amplify the target promoter region of Dusp1, n = 5 biological repetition. E After 24 h of normoxia or IH treated, PASMCs were cotransfected with si-Scramble or si-Nr1d1 and pGL3-Dusp1-promoter-Luci plasmid for 48 h. F Treated PASMCs were cotransfected with Nr1d1 plasmid and pGL3-m_wtDusp1-promoter-Luci (WT) or pGL3-m_mutDusp1-promoter-Luci plasmid (Mut) for 48 h, n = 5 biological repetition. G Co-IP of Nr1d1 in PASMCs was performed to confirm the interaction between Nr1d1 with corepressor NCoR1-HDAC3 complex. H Immunoblot images and the quantitative analysis of Dusp1 protein expression from control and IH-treated PASMCs. I Cellular immunofluorescence double labeling reveals Dusp1 expression in PASMCs. Alexa Fluor-488 labels α-SMA as green, Alexa Fluor-594 labels Dusp1 as red, and DAPI counterstains the nuclei in blue, scale bar = 5 µm. Data are shown as mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001.

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