Fig. 6: Silencing Dusp1 reverses the protective effects conferred by Nr1d1 deficiency on mitochondrial function. | Cell Death Discovery

Fig. 6: Silencing Dusp1 reverses the protective effects conferred by Nr1d1 deficiency on mitochondrial function.

From: Nr1d1 inhibition mitigates intermittent hypoxia-induced pulmonary hypertension via Dusp1-mediated Erk1/2 deactivation and mitochondrial fission attenuation

Fig. 6

A Representative flow cytometry images of Mito-SOX (red) staining in three PAMSCs groups (IH, IH+si-Nr1d1, IH +si-Nr1d1+si-Dusp1). B Summarized OCR tracings in PASMCs in indicated groups (n = 4 independent experiments). C The Basal OCR, maximum respiration, and ATP production in indicated groups of PASMCs. D Image and relative quantitative analysis of mitochondrial membrane potential in various groups of PASMCs using the JC-1 kit, scale bar = 50 µm. E Representative confocal images of the mitochondrial network of PASMCs examined by MitoTracker Deep Red, and the number of individual mitochondria as well as the mean mitochondrial branch lengths were calculated, scale bar = 5 µm. Involved 4 biological replicates (N = 4). Data are shown as mean ± SD; *P < 0.05 verse IH group; #P < 0.05 verse IH+si-Nr1d1 group.

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