Fig. 7: Dusp1 regulates Drp1-mediated mitochondrial fission through the modulation of Erk1/2 phosphorylation. | Cell Death Discovery

Fig. 7: Dusp1 regulates Drp1-mediated mitochondrial fission through the modulation of Erk1/2 phosphorylation.

From: Nr1d1 inhibition mitigates intermittent hypoxia-induced pulmonary hypertension via Dusp1-mediated Erk1/2 deactivation and mitochondrial fission attenuation

Fig. 7

A Representative immunoblot images and quantitative analysis of mitochondria fusion and fission-associated proteins following IH or IH+Dusp1 overexpression treatment in PASMCs. B Immunoblot analysis and quantification of p-Drp1, Drp1 protein expression following IH, IH+si-Nr1d1, IH+si-Nr1d1+siDusp1 in PASMCs. C Cellular immunofluorescence images of p-Drp1 in indicated PASMCs. Alexa Fluor-488 labels α-SMA as green, Alexa Fluor-594 labels p-Drp1 as red, and DAPI counterstains the nuclei in blue, scale bar = 5 µm. D Representative immunoblot images and quantitative analysis of Dusp1, Drp1, p-Drp1, p-Erk1/2, Erk1/2 proteins expression in IH-treated PASMCs exposed in Dusp1 or PMA. E Confocal images of the mitochondrial network of indicated PASMCs examined by MitoTracker Deep Red, and the number of individual mitochondria as well as the mean mitochondrial branch lengths were calculated, scale bar = 5 µm. Involved 4 biological replicates (N = 4). Data are shown as mean ± SD; *P < 0.05 verse IH group; #P < 0.05 verse IH+si-Nr1d1 group.

Back to article page