Fig. 8: Dusp1 knockdown exacerbates CIH-induced PH by enhancing Erk1/2 and Drp1 phosphorylation. | Cell Death Discovery

Fig. 8: Dusp1 knockdown exacerbates CIH-induced PH by enhancing Erk1/2 and Drp1 phosphorylation.

From: Nr1d1 inhibition mitigates intermittent hypoxia-induced pulmonary hypertension via Dusp1-mediated Erk1/2 deactivation and mitochondrial fission attenuation

Fig. 8

A Agarose gel electrophoresis results of Dusp1 knockout mice. B6 is the negative control and N is the blank control. B Immunoblot images and quantitative analysis of Dusp1, Drp1, p-Drp1, p-Erk1/2, Erk1/2 proteins expression in mice lung tissue of control group, CIH group, and CIH+Dusp1−/− group. C Histological evaluation of mouse lung tissues containing peripheral pulmonary vascular in indicated groups mouse through H&E staining, α-SMA immunohistochemistry, and Masson staining, scale bar = 10 µm. D RVSP in three groups of mice. E Relative quantitative analysis of RVSP in three groups of mice. F Fulton index in indicated groups of mice. G Quantification of vascular medial wall thickness in image C. H The ratio of non-muscularized, partially muscularized, and fully muscularized pulmonary arterioles in CIH-induced PH mice. Involved 6 biological replicates (N = 6). Data are shown as mean ± SD; *P < 0.05 verse Control group; #P < 0.05 verse CIH+ Dusp1−/− group.

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