Fig. 2: Fibro-adipose accumulation in co-culture models. | Cell Death Discovery

Fig. 2: Fibro-adipose accumulation in co-culture models.

From: Cardiomyocyte and stromal cell cross-talk influences the pathogenesis of arrhythmogenic cardiomyopathy: a multi-level analysis uncovers DLK1-NOTCH pathway role in fibro-adipose remodelling

Fig. 2

A, C Representative images of immunostaining for Col1A1 (green), Nile Red (red), and alpha-Actinin (white), markers of myofibroblasts, adipocytes and cardiomyocytes respectively. Nuclei are stained with Hoechst 33342 (blue). Magnification: 40X; scale bar: 50 μm. B, D Quantification of the images shown as fold change with respect to HC co-culture model (HC CM_HC cMSC) with p-values from One-way ANOVA and Tukey’s post-test are indicated in each panel. For Col1A1 staining: n = 9 for HC CM_HC MSC; n = 9 for ACM CM_ACM MSC; n = 9 for ACM CM_HC MSC; n = 9 for HC CM_ACM MSC. For Nile Red staining n = 8 for HC CM_HC MSC; n = 9 for ACM CM_ACM MSC; n = 8 for ACM CM_HC MSC; n = 8. Three independent differentiations were used for both Col1A1 and Nile Red staining. Data information: mean ± SEM. **P < 0.01 ****P ≤ 0.0001.

Back to article page