Fig. 4: Anakinra alleviates the phenotype of EH mice and reduces the level of glutamate in the inner ear by downregulating GLS. | Cell Death Discovery

Fig. 4: Anakinra alleviates the phenotype of EH mice and reduces the level of glutamate in the inner ear by downregulating GLS.

From: IL-1β promotes glutamate excitotoxicity: indications for the link between inflammatory and synaptic vesicle cycle in Ménière’s disease

Fig. 4

a qRT-PCR for Gls expression in HEI-OC1 cells (n = 3) stimulated with IL-1β (10, 25, 50 ng/mL) for 24 h. b Representative images of western blotting detecting GLS in HEI-OC1 cells (n = 3) stimulated with IL-1β (10, 25, 50 ng/mL) for 24 h. GAPDH was used as a loading control. c Glutamate level in HEI-OC1 cells (n = 6) stimulated with IL-1β (10, 25, 50 ng/mL) for 24 hours. d qRT-PCR for Gls expression in HEI-OC1 cells (n = 3) treated with or without siGls. e Western blotting to analyze GLS expression in HEI-OC1 cells (n = 3) treated with or without siGls. GAPDH was used as a loading control. f Glutamate level in HEI-OC1 cells (n = 6) stimulated with IL-1β and/or siGls for 24 h. g Glutamate level in serum and inner ear of mice (n = 6) untreated or pretreated treated with Anakinra (10 mg/kg, i.p.) and challenged with LPS (10 mg/kg, p.a.) or equivalent saline for 3 consecutive days. h Transmission electron microscopy showing the ultrastructure of VO in mice (n = 3) untreated or pretreated treated with Anakinra and challenged with LPS or equivalent saline. Arrows mark the synapse. Scale bars,500 nm. i qRT-PCR for Gls expression in mice (n = 5) untreated or pretreated treated with Anakinra and challenged with LPS or equivalent saline. *p < 0.05, **p < 0.01, ***p < 0.001.

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