Fig. 1: RRM1 enhances the radiation resistance of tumor cells by promoting HR repair. | Cell Death Discovery

Fig. 1: RRM1 enhances the radiation resistance of tumor cells by promoting HR repair.

From: RRM1 promotes homologous recombination and radio/chemo-sensitivity via enhancing USP11 and E2F1-mediated RAD51AP1 transcription

Fig. 1

A, B Western blot analysis of RRM1 expression in HeLa cells treated with ionizing radiation and DNA damage drugs. C Detection of DNA damage markers γH2AX before and after ionizing radiation in HeLa wild-type and RRM1 knockdown cell lines. D Colony-forming assay to evaluate the colony-forming ability of HeLa wild-type and RRM1 knockdown cells before and after ionizing radiation. E Flow cytometry analysis to determine the proportion of GFP-positive cells indicative of HR repair (DR-GFP) or NHEJ repair (EJ5-GFP) in HeLa wild-type and RRM1 knockdown cells. F Sensitivity assay showing the response of HeLa wild-type cells and RRM1 knockdown cells to the PARP inhibitor olaparib after irradiation. G Chromatin separation experiment assessing the chromatin recruitment of RAD51 in HeLa wild-type and RRM1 knockdown cells. H Immunofluorescence staining to visualize the formation of RAD51 foci in HeLa wild-type and RRM1 knockdown cells after DNA damage.

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