Fig. 2: RRM1 promotes the transcription of RAD51AP1. | Cell Death Discovery

Fig. 2: RRM1 promotes the transcription of RAD51AP1.

From: RRM1 promotes homologous recombination and radio/chemo-sensitivity via enhancing USP11 and E2F1-mediated RAD51AP1 transcription

Fig. 2

A Heatmap comparison of differentially expressed genes in HCT116 and A549 cells between low and high RRM1 expression groups. B Measurement of RAD51AP1 mRNA expression before and after ionizing radiation (IR) in HeLa wild-type and RRM1 knockdown cells. C Evaluation of the effect of RRM1 knockdown on RAD51AP1 protein levels. D Analysis of RAD51AP1 protein levels over time in HeLa wild-type and RRM1 knockdown cells after IR. E Immunofluorescence staining of RAD51AP1 to assess the impact of RRM1 knockdown on RAD51AP1 protein expression. F Transfection of HEK293T cells with an RRM1-carrying plasmid followed by Western blot analysis to examine changes in RAD51AP1 protein levels. G Detect the RAD51AP1 protein content at regular intervals of WT and RRM1 knockdown HeLa cells treated with CHX. H Transfer the plasmid carrying RAD51AP1 into HeLa cells with knockdown of RRM1 and assay their colony-forming ability. Compare this with the colony-forming ability of HeLa wild-type and RRM1 knockdown cells following IR treatment.

Back to article page