Fig. 3: RRM1 promotes mRNA expression of RAD51AP1 dependent on E2F1. | Cell Death Discovery

Fig. 3: RRM1 promotes mRNA expression of RAD51AP1 dependent on E2F1.

From: RRM1 promotes homologous recombination and radio/chemo-sensitivity via enhancing USP11 and E2F1-mediated RAD51AP1 transcription

Fig. 3

A Transfer of control plasmids NC and E2F1 siRNA separately, followed by detection of RAD51AP1 protein expression in HEK293T cells. B Detection of E2F1 protein expression in HeLa wild-type and RRM1 knockdown cell lines. C Detection of E2F1 protein levels in wild-type and RRM1 knockdown cells at intervals after adding the protein synthesis inhibitor CHX. D Evaluation of the effect of proteasome inhibitor MG132 on E2F1 protein levels in HeLa RRM1 knockdown cell lines treated with CHX. E Detection of ubiquitination levels of E2F1 in HeLa wild-type and RRM1 knockdown cell lines, respectively. F Transfer siE2F1 into RRM1 knockdown HeLa cells and detect the transcriptional changes of RAD51AP1. G Immunoprecipitation of E2F1 and RRM1 in HeLa wild-type cells using anti-RRM1 antibody and E2F1 antibody, respectively, followed by detection of the protein levels of E2F1 and RRM1 by Western blot.

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