Fig. 5: RRM1 promotes the binding of USP11 to NPC. | Cell Death Discovery

Fig. 5: RRM1 promotes the binding of USP11 to NPC.

From: RRM1 promotes homologous recombination and radio/chemo-sensitivity via enhancing USP11 and E2F1-mediated RAD51AP1 transcription

Fig. 5

A Nuclear-cytoplasmic separation analysis was performed on HeLa wild-type cells at certain intervals after 5 Gy irradiation to detect the expression of RRM1 in the cytoplasm and nucleus. B HeLa cells were treated with 5 Gy irradiation, and the protein levels of LaminB1 and NUP50 co-precipitated with anti-RRM1 antibody were detected by Western blot at different time points. C After treatment of HeLa cells with 5 Gy irradiation, the distribution of RRM1 and NUP50 at different time points after irradiation was observed under a fluorescence microscope. D After treatment of cells with 5 Gy irradiation, the distribution of RRM1 and LaminB1 at different time points after irradiation was observed under a fluorescence microscope. E, F Nuclear-cytoplasmic separation analysis was performed on HeLa wild-type cells at certain intervals after 5 Gy irradiation to detect the expression of RRM1 and USP11 in the cytoplasm and nucleus. The relative quantities of RRM1 and USP11 in the nucleus and internal reference H3 were plotted at each time point based on the grayscale values of WB bands. G The protein levels of several major nuclear pore complexes co-precipitated with anti-USP11 antibodies were detected in HeLa wild-type and RRM1 knockdown cells, with IgG as a negative control.

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