Fig. 6: The C-terminus of RRM1 is crucial for RRM1 to bind to USP11 and facilitate USP11’s entry into the nucleus. | Cell Death Discovery

Fig. 6: The C-terminus of RRM1 is crucial for RRM1 to bind to USP11 and facilitate USP11’s entry into the nucleus.

From: RRM1 promotes homologous recombination and radio/chemo-sensitivity via enhancing USP11 and E2F1-mediated RAD51AP1 transcription

Fig. 6

A Structural sequence of RRM1 based on literature. B Schematic diagram of the RRM1 truncated body model with missing sequences. C-D Potential interaction sites between RRM1, USP11, and LaminB1 predicted using online protein structure prediction software. E Transfection of HEK293T cells with various RRM1 constructs, followed by treatment with 5 Gy irradiation. Immunoprecipitation with anti-flag antibody and analysis of the precipitate by immunoblotting with RRM1, USP11, LaminB1, Flag, and Actin. F Comparison of expression levels of E2F1 and RAD51AP1 proteins in HeLa cells transfected with different RRM1 constructs compared to wild-type cells. G Detection of the protein level of E2F1 co-precipitated with anti-USP11 antibody immunoblotting after transfection with full-length and truncated RRM1 constructs. H Analysis of the distribution of RRM1 and its truncated bodies within HEK293T cells after 5 Gy treatment using fluorescence immunoassay. I Detection of E2F1 co-precipitated with Ub after transfection with full-length and truncated RRM1 constructs. J Observation of colony-forming ability of HEK293T cells under irradiation and non-irradiation conditions after transfection with full length and truncated RRM1 constructs. K CHX treatment of HEK293T cells carrying full-length and truncated RRM1 constructs, followed by detection of the protein levels of E2F1 by Western blotting at different time points.

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