Fig. 3: HA induces enhanced lysosome biogenesis and their rupture in MM. | Cell Death Discovery

Fig. 3: HA induces enhanced lysosome biogenesis and their rupture in MM.

From: Hexamethylene amiloride induces lysosome-mediated cell death in multiple myeloma through transcription factor E3

Fig. 3

A KEGG analysis of the up-regulated genes. B Heatmap show Log2 fold change of the significantly differentially expressed genes in lysosomal pathway. C RT-qPCR of some significantly differentially expressed genes in lysosomal pathway of RPMI-8226 (left) and U266 (right). Cell were treated with HA at 10 μM for 48 h. D, E Lysotracker staining of RPMI-8226 (D) and U266 (E) after treated HA at 10 μM for 48 h. Right panel, mean fluorescence intensity statistics. F, G Western Blot detect the expression of LAMP1 of RPMI-8226 (F) and U266 (G) treated HA at 10 μM for 48 h and statistical analysis of LAMP1 expression, normalized by tubulin. H Transmission electron microscope shows the lysosome when RPMI-8226 treated HA for 48 h, yellow arrow: lysosome. I, J Acridine Orange staining of RPMI-8226 (I) and U266 (J) after treated HA at 10 μM for 48 h. Right panel, mean fluorescence intensity statistics. Data are presented as the mean ± SD of at least three independent experiments, and comparisons were evaluated by two-tailed Student’s t test. *, P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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