Fig. 2: Rian/MEG8 expression decreases with age.
From: MEG8 as an antagonistic pleiotropic mechanism in breast cancer
A Expression levels of the Dlk1-Dio3 cluster genes by a microarray analysis in the breast tissue of three different strains of mice, C57BL/6 J, SAMP6 and AKRJ. We used 3/4-, 16- or 93-weeks (w) old animals, selecting three females and three males of each condition. B Expression levels of Rian in the microarray analysis. Here, we independently show the results of females and males (n = 3 in each condition). C Measurement of Rian expression levels by RT-qPCR using the same samples of the microarray. D, E Measurement of MEG8/SNORD112/SNORD113-4 expression levels by RT-qPCR using human blood samples from a cohort of patients with different age. Data were analyzed using Student’s t-test. *p < 0.05, ** p < 0.01, *** p < 0.001.
