Fig. 3: Overexpression of MEG8 induces senescence in normal cells.
From: MEG8 as an antagonistic pleiotropic mechanism in breast cancer
A Growth curve of control and MEG8 overexpressing mouse embryonic fibroblasts (MEFs). B Percentage of MEFs with senescence associated (SA) – β-galactosidase activity after Xgal staining. Representative images are shown. C Measurement of MEG8, Rian, p16, p21, p15 and p19 expression levels by RT-qPCR in control and MEG8 overexpressing MEFs. D Validation of MEG8 overexpression in MCF10A cell line by RT-qPCR. E Growth curve of MCF10A control and MEG8 cell lines. F Measurement of p21 expression levels by RT-qPCR in MCF10A control and MEG8 cell lines. G Percentage of MCF10A cells with senescence associated (SA) – β-galactosidase activity after Xgal staining. Representative images are shown. H Measurement of apoptosis in MCF10A control and MEG8 cell lines through FACS by using Annexin V and propidium iodide stainnings. We consider early-apoptotic cells (Annexin positive/propidium iodide negative cells), necrotic cells (Annexin negative/propidium iodide positive cells), and apoptotic cells (Annexin positive/propidium iodide positive cells). All the figures show the average of three independent experiments performed in triplicate. Data were analyzed using Student’s t-test. *p < 0.05; **p < 0.01; ***p < 0.001.
