Fig. 5: E2 downregulated ATF4 and protected MPP+-treated BV2 cells. | Cell Death Discovery

Fig. 5: E2 downregulated ATF4 and protected MPP+-treated BV2 cells.

From: 17β-estradiol alleviated ferroptotic neuroinflammation by suppressing ATF4 in mouse model of Parkinson’s disease

Fig. 5

A, B GSEA results based on the KEGG database for microglia from hippocampi of female and male mice treated with LPS. C Morphological changes of BV2 cells. D The ferroptosis was measured by flow cytometry using annexin V-FITC/7ADD staining. E Western blot showed the effects of E2 on COX-2, ATF4, and IL-17A levels in MPP+-treated BV2 cells. F Western blot showed the effects of ATF4 silencing on COX-2, ATF4, and IL-17A levels in MPP+ and E2-treated BV2 cells. G Western blot showed the effects of E2 on TLR4 and pNF-κB levels in MPP+-treated BV2 cells. H IHC confirmed TLR4 and pNF-κB expression in BV2 cells. I Western blot showed the effects of ATF4 overexpression on TLR4 and pNF-κB levels in MPP+ and E2-treated BV2 cells.

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