Fig. 4: Overexpression of PDSS2-Del2 enhances MST1 expression by promoting SKOR1 degradation.
From: Overexpression of PDSS2-Del2 in HCC promotes tumor metastasis by interacting with macrophages
A The protein levels of SKOR1 were detected using western blotting. B The RNA levels of SKOR1 were detected using qPCR. C, D The protein levels of SKOR1 were detected in PDSS2-Del2 overexpressed 8024 cells (C) and Huh7 (D) and control cells. Cells were treated with CHX at various time points. E The protein levels of SKOR1 were detected in cells treated with or without MG132. F Co-IP was performed with an anti-SKOR1 antibody, and polyubiquitylated SKOR1 was detected by ubiquitin antibody. G SMAD3 and p-SMAD3 in PDSS2-Del2 overexpressed HCC cells and control cells. H SMAD3 and p-SMAD3 in PDSS2-Del2 overexpressed HCC cells and control cells with or without SKOR1 overexpression. I The RNA levels of SMAD3 in SMAD3-KD cells. J The protein levels of MST1 in PDSS2-Del2 overexpressed HCC cells with or without SMAD3-KD. (**P < 0.01, ***P < 0.001).
