Fig. 6: Co-culture with PDSS2-Del2 overexpressed HCC cells promotes macrophages to secrete metalloproteinases by activating the PI3K/AKT pathway.
From: Overexpression of PDSS2-Del2 in HCC promotes tumor metastasis by interacting with macrophages
A Protein levels of AKT, p-AKT, PI3K, and C-RAF were determined in THP-1 cells co-cultured with CM from PDSS2-Del2 overexpressed HCC cells. B, C The RNA (B) and protein (C) levels of MMP9 and MMP2 were determined in THP-1 cells co-cultured with CM form PDSS2-Del2 overexpressed HCC cells and control cells. The MST1 inhibitor (40 nM) was added to the co-culture system (C). D The activities of metalloproteinases were detected by gel enzyme spectrometry in THP-1 cells co-cultured with CM form PDSS2-Del2 overexpressed HCC cells and control cells. E Mechanism by which PDSS2-Del2 overexpressed HCC cells interact with macrophages and enhance tumor cell metastasis.
