Fig. 2: Cdk14 is indispensable during bleomycin-induced lung regeneration. | Cell Death Discovery

Fig. 2: Cdk14 is indispensable during bleomycin-induced lung regeneration.

From: CDK14 regulates the development and repair of lung

Fig. 2

A Survival curve of Cdk14+/+ (n = 4) and Cdk14−/− (n = 4) after intratracheal injection of 0.3 mg/ml bleomycin 50 μl. Log-rank (Mantel-Cox) test was used to calculate the P value. B Representative histological morphology of Cdk14+/+ and Cdk14−/− mice lung stained by hematoxylin-eosin and sirius red. C Representative projected confocal images showing EMCN+ (green) blood vessels and NKX2.1+ (blue) lung epithelial progenitor cell and αSMA (red) labelled myofibroblasts in bleomycin-treated Cdk14+/+ and Cdk14−/− mice lung. Quantification about covered area of EMCN and αSMA and NKX2.1 labelled lung epithelial progenitor cell number in bleomycin-treated Cdk14+/+ and Cdk14−/− mice lung. All data are normalized to Cdk14+/+. Cdk14+/+ = 4；Cdk14−/− = 4. Error bars, mean \(\pm\) s.e.m. P values, t-test. D Representative projected confocal images showing RAGE+ (green), SFTPC+ (blue) and EdU+ cells in bleomycin-treated Cdk14+/+ and Cdk14−/− mice lung. Quantification about covered area of RAGE and SFTPC labelled AT2 cell number in bleomycin-treated Cdk14+/+ and Cdk14−/− mice lung. All data are normalized to Cdk14+/+. Cdk14+/+ = 4；Cdk14−/− = 4. Error bars, mean \(\pm\) s.e.m. P values, t-test. E Diagram depicting for CD11b+Gr-1+ cells in bleomycin-treated ctrl and Cdk14 KO mice lung. Quantification about the number of CD11b+Gr-1+ myeloid cells in the two groups. Cell frequencies show the percentage of target population in total cells. Cdk14 Ctrl = 6；Cdk14 KO = 6. Error bars, mean ± s.e.m. P values, t-test. F Diagram depicting for neutrophils (Ly6G+, CD11b+) in bleomycin-treated ctrl and Cdk14 KO mice lung. Quantification about the number of neutrophils in the two groups. Cell frequencies show the percentage of target population in total cells. Cdk14 Ctrl = 6；Cdk14 KO = 6. Error bars, mean ± s.e.m. P values, t-test. G Diagram depicting for interstitial macrophages (CD64+) and monocytes (CD64-) in bleomycin-treated ctrl and Cdk14 KO mice lung. Quantification about the number of interstitial macrophages and monocytes in the two groups. Cell frequencies show the. percentage of target population in total cells. Cdk14 Ctrl = 6；Cdk14 KO = 6. Error bars, mean ± s.e.m. P-values, t-test. H Diagram depicting for B cells (B220+, CD19+) in bleomycin-treated ctrl and Cdk14 KO mice lung. Cell frequencies show the percentage of target population in total cells. Quantification about the number of B cells in the two groups. Cdk14 Ctrl = 6；Cdk14. KO = 6. Error bars, mean ± s.e.m. P values, t-test.

Back to article page