Fig. 2: IDO1 suppressed lipid peroxidation generation and ferroptosis in GBM cells.
A Colony formation assays were used to detect the colony seeding ability of the effects of IDO1 overexpression (oeIDO1) in U87 and LN229 cells (left), quantification of relative clones (right). B Cell death rate was determined by Propidium Iodide (PI) staining flow cytometry in U87 cells with IDO1 overexpression. C Western blotting was used to detect the expression of PARP1, cleaved-PARP1 (c-PARP1), Caspase-7, and Caspase-3 in IDO1 overexpressed U87 cells, LE: long exposure, SE: short exposure (n = 3 biologically replicates). D The cell viability of U87 cell treated with Erastin (20 μM), ML-210 (40 μM), Etoposide (10 μM), Cisplatin (20 μM), Emodin (40 μM), Temozolomide (400 μM) for 72 h. E The cell viability curve of U87 and LN229 cells treated with 0, 10, 20, 40, and 80 μM Erastin for 72 h. ROS was measured by flow cytometry using DCFH-DA staining in U87 (F) and LN229 (G) cells after treatment with or without Erastin. Lipid peroxidation was measured by flow cytometry using C11-BODIPY581/591 staining in U87 (H) and LN229 (I) cells treated with or without Erastin. J Representative images of typical ferroptosis mitochondrial morphology in U87 cells. Green arrows: shrunken and heavy stained mitochondria; red arrows: ruptured mitochondrial membrane; N: nucleus, scale bar = 2 μm or 500 nm. K The statistic results of mitochondrial length in the Vector (n = 6) and oeIDO1 (n = 6) U87 cell group. The data are represented as the mean ± standard deviation (SD); *p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant.
