Fig. 3: IDO1 regulation of ferroptosis in GBM cells depends on SLC7A11 expression. | Cell Death Discovery

Fig. 3: IDO1 regulation of ferroptosis in GBM cells depends on SLC7A11 expression.

From: IDO1 inhibits ferroptosis by regulating FTO-mediated m6A methylation and SLC7A11 mRNA stability during glioblastoma progression

Fig. 3

Cystine levels (A) and glutathione (GSH) levels (B) were detected in U87 and LN229 cells with IDO1 overexpression. C RT-qPCR was used to detect the mRNA levels of IDO1, SLC7A11 and GPX4 in U87 and LN229 cells with IDO1 overexpression (n = 3 biologically replicates). D Western blotting was used to detect the protein expression of IDO1, SLC7A11, and GPX4 in U87 and LN229 cells with IDO1 overexpression (n = 3 biologically replicates). E Immunofluorescence (IF) staining showed the protein expression and subcellular distribution of SLC7A11 in U87 and LN229 cells with IDO1 overexpression, scale bar = 20 μm. F IHC staining was used to detect the expression of SLC7A11 in GBM tissues and adjacent normal tissues from the clinical GBM specimens (n = 12), scale bar = 100 μm. G Lipid peroxidation levels were measured by C11-BODIPY581/591 staining flow cytometry after SLC7A11 knockdown in U87 cells. The data are represented as the mean ± standard deviation (SD); *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant.

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