Fig. 4: mtROS are essential for maintaining mitochondrial mass in colorectal cancer cells under acidic microenvironment. | Cell Death Discovery

Fig. 4: mtROS are essential for maintaining mitochondrial mass in colorectal cancer cells under acidic microenvironment.

From: Autophagic flux-lipid droplet biogenesis cascade sustains mitochondrial fitness in colorectal cancer cells adapted to acidosis

Fig. 4

A MitoSOX distribution was measured by flow cytometry in CRC and CRC-AA cells. B Mitochondrial content was quantified by flow cytometry in CRC and CRC-AA cells treated with mitoQ (125 nM) from 12 h to 24 h detected by Mito-tracker. C Western blot analysis of OXPHOS protein level in CRC and CRC-AA cells treated with mitoQ (125 nM or 500 nM) from 24 h. COX IV was used as a loading control. D Apoptotic levels of CRC and CRC-AA cells after treatment with mitoQ (125 nM) from 12 h to 48 h. E Scheme for treatment paradigm of subcutaneous tumor xenografts. Because HCT15-AA cells are less proliferative than their parental cells, they were injected into nude mice 7 days earlier than their parental cells. 12 days later, the tumor-bearing mice were randomized into two groups: vehicle only (DMSO, n = 4), mitoQ only (500 nM, n = 4, po.). Tumor weights were measured at day 34. F Tumor xenografts formed by HCT15-AA are more sensitive to mitoQ. G Representative images of immunohistochemistry staining using Ki-67 antibody in tumors for each group. Scale bar: 50 μm. *p < 0.05, **p <0.01, ***p < 0.001, ns indicates p > 0.05.

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