Fig. 1: Robust generation of ready-to-use cryopreserved motor neurons (MNs) from induced pluripotent stem cells (iPSCs). | Cell Death Discovery

Fig. 1: Robust generation of ready-to-use cryopreserved motor neurons (MNs) from induced pluripotent stem cells (iPSCs).

From: Rapid iPSC-derived neuromuscular junction model uncovers motor neuron dominance in amyotrophic lateral sclerosis cytopathy

Fig. 1

A Procedure of MN differentiation and cryopreservation. B Immunocytochemistry (ICC) staining of differentiated cells for neural stem cell (NSC) markers PAX6, SOX1, NES and NCAD; motor neuron progenitor (MNP) marker OLIG2; and MN marker ISL1 and HB9 in differentiated cells on day 10 and day 14. C ICC for MN markers HB9 and NF on day 28 of differentiation. D ICC for MN markers HB9, ISL1, CHAT, and neuronal marker NF, PSD95 and SYP on day 4 of thawing. Green arrowheads indicate the green fluorescence cells, red arrowheads indicate the red fluorescence cells, and yellow arrowheads indicate cells positive for both green and red fluorescence. E Calculation of the expression ratio of relevant markers in DAPI+ cells on day 4 post-thaw. 6 images from 3 independent experimental replicates were analyzed, with each data point representing the mean value from a single replicate. The procedure in (A) was generated using Microsoft PowerPoint. SFEB serum-free embryoid body, NI neural induction, CHIR CHIR99021, SB SB431542, FGFb FGF-basic, LDN LDN193189, CpdE Compound E, and RA retinoic acid. Scale bars: 50 μm (B, C, D).

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