Fig. 2: Generation of myoblast cryopreservation stocks and further skeletal muscle (SKM) maturation from induced pluripotent stem cells (iPSCs).

A Differentiation procedure of skeletal muscles (SKM). B Cell morphology during SKM differentiation, amplification, and maturation. C–H Immunocytochemistry (ICC) of differentiated cells for SKM markers MHC, MYOD, MYOG, and TTN on day 12 of SKM maturation; Arrowheads indicate the multinuclear morphology in (E, H). The procedure in (A) was generated using Microsoft PowerPoint. Scale bars: 300 μm (B); 100 μm (C, F); 20 μm (D, E, G, H).