Fig. 5: Volume of skeletal muscles (SKMs), motor neurons (MNs), and acetylcholine receptors (AChRs) in the neuromuscular junction (NMJ) model derived from the cross-coculture of SOD1G85G and SOD1G85R MNs and SKMs. | Cell Death Discovery

Fig. 5: Volume of skeletal muscles (SKMs), motor neurons (MNs), and acetylcholine receptors (AChRs) in the neuromuscular junction (NMJ) model derived from the cross-coculture of SOD1G85G and SOD1G85R MNs and SKMs.

From: Rapid iPSC-derived neuromuscular junction model uncovers motor neuron dominance in amyotrophic lateral sclerosis cytopathy

Fig. 5

A Diagram of the cross-coculture of SOD1G85R and SOD1G85G MNs and SKMs. B 3D confocal images of immunocytochemistry (ICC) staining for the SKM marker TTN; neuronal marker NF; and NMJ markers SV2 and AChR. C–G Calculations of the volume of SKMs (C), MNs (D), and the ratios of MN/SKM (E), AChR/SKM (F), and AChR/MN (G) in day 9 cross-coculture NMJ models. Five (SOD1G85G MN + SOD1G85G SKM), 4 (SOD1G85R MN + SOD1G85G SKM), 8 (SOD1G85G MN + SOD1G85R SKM) and 6 (SOD1G85R MN + SOD1G85R SKM) images from 3 independent experimental replicates were analyzed, with each data point representing the mean value from a single replicate. The one-way analysis of variance with Tukey’s post hoc test was used. The schematic in (A) was generated using Microsoft PowerPoint. Scale bar: 20 μm in confocal 3D images; and 5 μm in 2D image slices.

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