Fig. 1: METTL1 is overexpressed in cSCC tumors and cell lines. | Cell Death Discovery

Fig. 1: METTL1 is overexpressed in cSCC tumors and cell lines.

From: METTL1 coordinates cutaneous squamous cell carcinoma progression via the m7G modification of the ATF4 mRNA

Fig. 1

A The protein levels of METTL1 in normal specimens (n = 7) and cSCC tumors (n = 36) with different tumor grade were detected by IHC. B The samples highlighted in red in A were magnified. The intensity optical density (IOD) for each sample was calculated. C The mRNA levels of METTL1 in normal specimens and cSCC tumors were detected by qRT-PCR. D, E The expression of METTL1 in HaCaT keratinocytes and cSCC cell lines (HSC-1 and A431) were detected by western blot and qRT-PCR, respectively. β-Tubulin was used for the normalization control. The relative protein level of METTL1 was calculated. F The m7G methylation levels in total RNA and decapped mRNA from cell lines were detected by dot blot assay. Data are shown as mean ± SEM. ***P < 0.001, n = 6. MB methylene blue.

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