Fig. 2: METTL1 knockdown significantly represses cSCC cells survival.

A, B Western blot and qRT-PCR showed METTL1 was successfully knocked down by shMETTL1 at protein and mRNA levels. β-Tubulin was used for the normalization control. C The m7G methylation levels in METTL1-knockdown cSCC cell lines were detected by dot blot assay. D Measurements of cell viability by CCK8 assay (n = 6). E EdU staining assay was used to assess cell proliferation (n = 3). The relative ratio of EdU⁺ cells were calculated. F The proportions of EdU⁺ cells were detected by flow cytometry (n = 3). G Measurements of cell proliferation by colony formation assay (n = 3). The colony number was calculated. H The cell apoptosis was determined by Annexin V/PI double staining (n = 6). I, J The protein and mRNA levels of BCL-2 and BAX were detected by western blot and qRT-PCR (n = 6), respectively. β-Tubulin was used for the normalization control. K, L TUNEL staining of HSC-1 and A431 cells was performed (n = 3). Data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.